Microrheology with Optical Tweezers: Principles and Applications. Manlio Tassieri

Microrheology with Optical Tweezers: Principles and Applications

ISBN: 9789814669184 | 350 pages | 9 Mb

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  • Microrheology with Optical Tweezers: Principles and Applications
  • Manlio Tassieri
  • Page: 350
  • Format: pdf, ePub, fb2, mobi
  • ISBN: 9789814669184
  • Publisher: Taylor & Francis
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Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to μs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)! Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies. The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the "nature of light," followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.

Micro-rheology of cells and soft matter with the NanoTracker™
underlying optical trapping, please refer to our application basic principles of their analysis using the integrated data Figure 4 Active micro-rheology. A trap   NanoTracker™ 2 - JPK Instruments AG
The NanoTracker™ 2 is an optical tweezers platform based on research-grade inverted optical from the experience of users in a wide range of applications. Thorlabs.com - Optical Tweezers Microscope Systems
For more details on this and other applications of optical tweezers, please see the Trapping of Dielectric Particles and Force Measurement; Microrheology Since each method relies on a different physical principle, the combined results  Microrheology with Optical Tweezers: Measuring the solutions
The advantages of the proposed microrheology method become evident, for instance, when it is adopted for measuring the molecular weight of  Encyclopedia of Nanotechnology - Trinity College Dublin
shows the principle of optical trapping in the Mie regime. Another technique, laser-tracking micro-rheology emerging applications for OT have led to various. Investigating the micro-rheology of the vitreous humor - IOPscience
Keywords: optical trapping, micro-rheology, vitreous humor. PACS numbers: Collagen, the principle protein constituent, is stabilized by the  Optical tweezers: wideband microrheology - IOPscience
Microrheology is a branch of rheology having the same principles as already extensive range of applications for which optical tweezers have been used, such   Microrheology with optical tweezers: data analysis
Since their first appearance in the 1970s [1], optical tweezers (OT) to be an invaluable tool for a variety of applications throughout temporal resolution, is one of the basic principles behind microrheology techniques [14, 

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